Causes and control strategies of pressure fluctuation in high-level biosafety laboratory

Analyses the physical significance of negative pressure in biosafety laboratory. Summarizes the main influencing factors of pressure fluctuation in high-level biosafety laboratory. Presents the technical requirements on the negative pressure gradient and the anti-interference ability in the national standard for biosafety laboratory. Elaborates common technical measures of pressure gradient control used in biosafety laboratory. Provides dynamic control strategies of pressure gradient in high-level biosafety laboratory. The analysis result of a case study shows that the control strategies can effectively solve the interference problem of the pressure fluctuation in the laboratory and ensure the directional flow of the laboratory.